It looks like it probably helped us, but it's hard to tell. At least one receiver stopped running his route I assume because the ref was blowing his whistle. It takes a bit longer for everyone else to understand the play was dead.

But I don't see anything before the snap that looks particularly concerning. I guess he just didn't like the play called with what the defense was showing? The defense after the timeout set up much differently. It would be interesting to hear more details though.